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. 2010 Jan 27;9:19. doi: 10.1186/1476-4598-9-19

Figure 1.

Figure 1

Nelfinavir induces cell death in leukemia cells. The human leukemia cell lines HL60, Jurkat, and IM9, and an ex vivo sample of human bone marrow cells (BMC) were treated for 48 h in cell culture with the indicated concentrations of nelfinavir and analyzed by a chemiluminescent ATP assay for cell survival (A), by annexin/PI staining [FL-1 (propidium iodide): 575 nm; FL-2 (FITC): 530 nm] for the occurrence of apoptosis and necrosis (B), and by phase contrast microscopy for morphological changes (C). For FACScan analysis and phase contrast microscopy, all cells were treated equally for 24 h with 8 μg/ml nelfinavir.