Figure 6.

Sorafenib enhances the efficacy of nelfinavir-induced cell death by mcl-1 downregulation. A) HL60 cells were treated with 5 μg/ml nelfinavir or 2 μg/ml sorafenib either alone or in combination, and analyzed after 24 h for the expression of apoptosis-related proteins by Western blot analysis. B) HL60 cells and ex vivo bone marrow cells (BMC) were incubated using the same conditions as in (A) and analyzed after 48 h for total cell survival by an ATP assay. C) HL60 and Jurkat cells were treated with either 5 μg/ml nelfinavir or 2 μg/ml sorafenib either alone or in combination, and the outer mitochondrial membrane potential was analyzed after 24 h by FACScan analysis.