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. 1997 Dec 9;94(25):13707–13712. doi: 10.1073/pnas.94.25.13707

Figure 1.

Figure 1

Specific in vivo interaction between Ldb1, LMO2, and SCL. (A) Schematic representation of two products of the Ldb1 gene, Ldb1a (421 residues), and Ldb1 (375 residues). The N terminus of Ldb1a is depicted as an ORF. LdbΔN corresponds to the LIM interaction domain, identified in the yeast two-hybrid system. (B) High affinity interaction between Ldb1 and LMO2, and complex formation between Ldb1, LMO2, and SCL/E2A. Cell extracts were coimmunoprecipitated with the indicated specific antisera and then immunoblotted using the antibody shown under each panel. G, glutathione S-transferase; F, Flag; H, HA; S, SCL; PI, preimmune sera; C, control mAb (α-HA). F-ΔN designates the Flag-tagged interaction domain of Ldb1. (C) Endogenous Ldb1 and LMO2 comigrate in gel filtration chromatography. Fractionation of nuclear extracts in a superose 6 column revealed comigration of Ldb1 and LMO2. The upper molecular mass band in the LMO2 Western blot (lanes 1, 10, 11) corresponds to a nonspecific cross-hybridizing protein.