Figure 3.

Expression of Ldb1 in developing and adult hemopoietic cells. (A) In situ hybridization of Ldb1 antisense RNA probe to mid-sagittal sections from E13.5 embryos. The arrow depicts fetal liver. (B) Immunofluorescence analysis demonstrating expression of Ldb1 in embryonic erythroblasts of fetal liver (E12.5). Cells were stained with α-Ldb1 peptide or preimmune sera. (C) Northern blot analysis of poly(A)⁺ RNA from murine hemopoieitic cell lines. The two Ldb1 transcripts are indicated in the upper panel. The control hybridization with a glyceraldehyde-3-phosphate dehydrogenase probe is shown in the lower panel. mØ, macrophage; Ery, erythroid; Ma, mast; Meg, megakaryocyte; T, T lymphoid.