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. 2010 Feb 23;11:128. doi: 10.1186/1471-2164-11-128

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Copyright ©2010 Liew et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR screening of bioinformatics-filtered dataset without contig information. (A) Schematic depicting the scenario whereby a contig from 1 species containing the gene of interest (shown as a hatched block) is aligned with the best corresponding hit contig from another species. In this case, the gene of interest is lost in 1 species due to missing sequence information while the adjacent flanking contig sequences are still present. (B) PFC0095c-PB000276.02.0 orthologs screened with (1)Pb, (2)Pc and (3)Py gDNA. PFL2450c-PC000344.03.0 orthologs screened with (4)Pb, (5)Pc and (6)Py gDNA. MAL8P1.310-PY06565 orthologs screened with (7)Pb, (8)Pc and (9)Py gDNA. PFB0645c-PB000193.00.0-PC000452.02.0 orthologs screened with (10)Pb, (11)Pc and (12)Py gDNA. PFF1480w(5'-end)-PB000730.00.0-PY03519 orthologs screened with (13)Pb, (14)Pc and (15)Py gDNA. PF13_0278-PC000860.02.0-PY04659 orthologs screened with (16)Pb, (17)Pc and (18)Py gDNA. (Legend: M = 100 bp DNA ladder)