Table 1.
Functional divergence between NCS subfamilies
| Subfamily (Class) | Subfamily (Class) | Type I θ (SE) | Type II θ (SE) | Positionsa |
|---|---|---|---|---|
| Frq/NCS-1 (A) | Recoverin (C) | 0.246 (0.089) | 0.342 (0.072) | 79, 102, 162 |
| Hippocalcin (B) | 0.858 (0.230) | 0.273 (0.046) | 79, 102, 162 | |
| KChIP1 (E) | 0.899 (0.233) | 0.415 (0.063) | 58, 79, 102, 138 | |
| KChIP2 (E) | NS | 0.348 (0.078) | 58 | |
| KChIP3 (E) | 0.594 (0.268) | NS | 138, 162 | |
| KChIP4 (E) | 0.561 (0.232) | 0.447 (0.057) | 58, 138, 162 | |
| VILIP2 (B) | NS | 0.298 (0.052) | 102 | |
| VILIP3 (B) | 0.829 (0.285) | - | 79, 102 | |
| Recoverin (C) | VILIP2 (B) | NS | 0.317 (0.076) | 102 |
| VILIP3 (B) | 0.563 (0.187) | NS | 102 | |
| GCAP1 (D) | 0.665 (0.069) | 0.480 (0.088) | 79, 161 | |
| GCAP2 (D) | 0.765 (0.076) | 0.501 (0.077) | 161, 161 | |
| KChIP1 (E) | 0.778 (0.090) | NS | 91 | |
| KChIP2 (E) | 0.867 (0.095) | NS | 79 | |
| KChIP3 (E) | 0.665 (0.070) | 0.547 (0.074) | 79, 161 | |
| KChIP4 (E) | 0.731 (0.100) | 0.500 (0.070) | 79, 161, 162 | |
| Hippocalcin (B) | KChIP1 (E) | NS | 0.455 (0.060) | 58 |
| KChIP2 (E) | NS | 0.430 (0.073) | 58 | |
| KChIP4 (E) | NS | 0.496 (0.055) | 58, 162 | |
| VILIP2 (B) | KChIP1 (E) | NS | 0.483 (0.060) | 161 |
| KChIP2 (E) | NS | 0.517 (0.066) | 161 | |
| KChIP3 (E) | NS | 0.416 (0.076) | 161 | |
| KChIP4 (E) | NS | 0.546 (0.055) | 161 | |
| GCAP3 (D) | GCAP2 (D) | NS | 0.498 (0.127) | 79 |
a Sites with changes between Frq1 and Frq2 that show significant shift in their evolutionary rate between NCS subfamilies (type I and type II sites are in regular and italic font, respectively). Positions are numbered relative to the D. melanogaster Frq1 protein. θ, ML estimate of the coefficient of functional divergence. SE, standard error. NS, non-significant.