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. Author manuscript; available in PMC: 2010 Mar 11.
Published in final edited form as: Biochim Biophys Acta. 2005 Nov 17;1760(2):233–244. doi: 10.1016/j.bbagen.2005.10.010

Fig. 3.

Fig. 3

Preincubation of mouse heart and brain cortex GCL under phosphorylating conditions decreases GCL enzyme activity. Homogenates of mouse brain cortex (A) and heart (B) were preincubated according to the procedures described in Materials and methods. GCL activity assays were usually for 15 min. The experiment was repeated three times and similar results were obtained. ***P <0.000001; **P <0.001. n =12 for each treatment, and HPLC analysis was conducted in duplicate. Control values for GCL activities were taken as the maximal GCL activity obtained without the addition of Mg2+ and ATP and were similar to the activities presented in the legend to Fig. 1.