Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Aug 14;20(4):898–911. doi: 10.1093/cercor/bhp156

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 The Authors

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Aberrant EPSP_KA tune PP-EPSPs to fire with a low temporal precision during synaptic integration. (A) Control: superimposed suprathreshold EPSPs evoked by stimulation (black arrow) of the PP in the outer one-third of the molecular layer alone (left) or following PI by stimulation in the inner one-third of the molecular layer (ISI = 40 and 30 ms; middle and right) in a granule cell from control rat. Note that PP-EPSPs evoked time-locked spikes in single-spike mode following PP stimulation alone (SD = 2.8 ms) and that the temporal precision of PP-EPSP-spike coupling was not modified during synaptic integration with PI-EPSP (SD = 1.2 ms, ISI = 40 ms; SD = 1.3 ms, ISI = 30 ms). Epileptic: same protocol in a DGC from an epileptic rat. Note that PP-EPSPs also evoked time-locked spikes in single-spike mode following PP stimulation alone (SD = 2.8 ms) but that the temporal precision of PP-EPSP-spike coupling is greatly decreased during synaptic integration with PI-EPSP_KA (SD = 10.2 ms, ISI = 40 ms; middle). This effect was fully reversed by 10 nM TTX superfusion (SD = 2.8 ms, ISI = 40 ms; right). (B) Superimposed supra-threshold EPSPs evoked by PP stimulations alone (left) or following somatic injection of a simulated EPSP_KA (sim; middle) in a granule cell from control rat. Note that PP-EPSPs evoked time-locked spikes in single-spike mode following PP stimulation alone (SD = 4.3 ms) but jittered spikes during synaptic integration with simulated EPSP_KA (SD = 11.3 ms, ISI = 30 ms). This effect was fully reversed by 10 nM TTX superfusion (SD = 1.6 ms, ISI = 30 ms; right). (C) Same as in A-epileptic in more physiological conditions (i.e., in ACSF without any drug and in cell-attached configuration). Note that PP stimulation also evoked time-locked spikes in single-spike mode in these conditions (SD = 0.8 ms, ISI = 30 ms; right) and jittered spikes following PI stimulation (SD = 4.0 ms; ISI = 30 ms; middle). This effect was fully reversed by 10 nM TTX superfusion (SD = 1.0 ms, ISI = 30 ms; right). (D) Bar graph of the mean EPSP-spike latency standard deviation (E–S SD) for PP stimulation alone (PP, white), PP stimulations following PI stimulations (PP + PI; grey) or PP stimulations following PI stimulations in the presence of TTX 10 nM (PP + PI in TTX, black) for DGCs from control rats (n = 8 cells; P > 0.05; left), epileptic rats (n = 11 cells for PP and PP + PI, and n = 5 for PP + PI in TTX; **P < 0.01; middle) and simEPSPKA (n = 10 cells for PP and PP + simEPSP_KA, and n = 8 for PP + simEPSP_KA in TTX; **P < 0.01; right).