Skip to main content
. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: J Virol Methods. 2009 Nov 24;164(1-2):43–50. doi: 10.1016/j.jviromet.2009.11.023

Figure 2. Transient expression of VSV G following electroporation.

Figure 2

(A) Vero cells were harvested at 24 (lanes 1–3) or 72 (lanes 4–7) h post-electroporation (EP) with expression plasmids (Fig. 1B) containing various forms of the VSV G gene (native Gin gene, lanes 1 and 4; Gene Optimized Gin, lanes 2 and 5; RNA Optimized VSV Gin, lanes 3 and 6), after which protein extracts were prepared for analysis by Western blotting. G protein was detected with a G-specific monoclonal antibody (Roche). Extract prepared from Vero cells infected with a recombinant propagation-competent VSVin was used as a positive control (Lanes 8). (B) The description of this experiment is similar to Part A, except that the effect of RNA optimization on expression of both the Gin and Gnj genes was evaluated. Gnj was detected with rabbit anti-VSVnj polyclonal antiserum.