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Representative images of NAc sections immunostained for Fos (red; a, d, g, j) and pERK (green; b, e, h, k) of animals of each experimental group: No Sex+Sal (a, b, c), Sex+Sal (d, e, f), No Sex+Meth (g, h, i), and Sex+Meth (j, k, l). Right panels are merged images illustrating co-localization of Fos and pERK. Arrows indicate dual labeled cells. Scale bar indicates 50 μm.
