Table 2.

Overview of mating-induced Fos and Meth-induced pERK expression in brain areas where sex and drugs activate non-overlapping neural populations.

Brain areas	Group	Fos	pERK	Dual	% Dual/pERK
Amygdala
CeA	Sal10	8.1 ± 2.0	33.7 ± 6.1	3.3 ± 0.8	11.1 ± 3.1
	Sex+Sal10	22.9 ± 2.7*	31.0 ± 5.7	13.9 ± 2.1*	43.2 ± 4.3
	Meth10	10.3 ± 2.2	95.3 ± 8.3#	5.1 ± 1.1	5.5 ± 1.6
	Sex+Meth10	21.6 ± 5.9*	77.6 ± 6.6#	17.3 ± 4.6*	20.7 ± 4.6
MEApd	Sal10	72.1 ± 13.5	40.2 ± 7.2	5.1 ± 1.9	11.4 ± 2.7
	Sex+Sal10	295.3 ± 48.1*	47.3 ± 6.7	13.8 ± 2.5*	29.4 ± 1.1
	Meth10	99.1 ± 6.4	84.5 ± 11.1#	9.1 ± 1.4	12.2 ± 2.2
	Sex+Meth10	403.5 ± 3.5*#	56.5 ± 7.1	11.4 ± 0.5	21.3 ± 3.9
BNST
BNSTpl	Sal10	19.9 ± 3.3	13.9 ± 5.9	2.5 ± 1.0	15.2 ± 5.4
	Sex+Sal10	116.8 ± 14.9*	6.8 ± 1.1	3.3 ± 0.8	49.3 ± 9.6
	Meth10	23.1 ± 1.7	35.4 ± 4.1#	2.9 ± 0.6	8.6 ± 1.2
	Sex+Meth10	113.3 ± 16.5*	20.6 ± 4.7*	4.9 ± 1.1	24.5 ± 10.5
mPFC
PL	Sal10	69.5 ± 13.4	31.5 ± 2.2	5.9 ± 2.1	17.9 ± 6.1
	Sex+Sal10	168.6 ± 32.4*	28.3 ± 1.6	12.0 ± 1.4*	41.7 ± 4.9
	Meth10	70.9 ± 11.9	49.7 ± 2.4#	8.1 ± 1.6	16.4 ± 3.3
	Sex+Meth10	148.1 ± 9.6*	32.3 ± 3.5*	13.0 ± 1.4	41.4 ± 5.9
IL	Sal10	59.8 ± 7.9	31.9 ± 2.0	5.2 ± 1.6	15.8 ± 4.4
	Sex+Sal10	129.8 ± 20.5*	23.5 ± 2.9	9.3 ± 2.3	39.4 ± 3.6
	Meth10	62.9 ± 9.0	47.6 ± 2.7#	6.1 ± 1.5	12.8 ± 3.3
	Sex+Meth10	108.5 ± 12.1*	30.1 ± 2.6*	8.3 ± 1.6	27.8 ± 3.7

Mean numbers ± of single and dual labeled neurons for pERK and Fos, as well as percentage of Meth activated neurons co-activated by sexual behavior are listed for each brain area for all four experimental groups.

^*indicate significant differences from unmated males of the same saline or Meth injected group (p < 0.05);

^#indicates significant differences from saline injected groups of the same sex or no sex treatment (p < 0.05).