Both DEX and plasmid DNA were continuously released from NPs for up to 14 days in PBS at 37 °C. Release kinetics of DEX NPs on surface (A) was identical to 10 mg of DEX NPs in 10 ml bulk PBS suspension (B). Due to the low density of DNA NPs on surface, plasmid DNA release kinetics from DNA NP coating was not directly measured. Plasmid DNA release was investigated only in bulk PBS suspension (C). (-○- 7 wt% DEX NP coatings, -▲- 7 wt% DEX NPs in bulk PBS suspension, -△- 0.2 wt% DNA NPs in bulk PBS suspension, n=3) For symbols without error bars, the standard deviation of the measurements was smaller than the size of the symbol.