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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biomaterials. 2010 Feb 10;31(13):3631–3642. doi: 10.1016/j.biomaterials.2010.01.048

Figure 6. Comparison of Macrophage Cell Response with Matrix Coatings.

Figure 6

(A) The anti-inflammatory drug was released directly into cell culture for 7 days from DEX matrix coated probes. 15.3 ± 0.1 μg and 154.6 ± 6.3 μg of DEX was released from 10 wt% and 50 wt% matrix coatings respectively, which corresponded to near 90% drug delivery efficiency for both in comparison with unencapsulated DEX of same amount. (B) This value was compared with the % drug delivery efficiency obtained by DEX NP coating. (C) 28.5 ± 0.3 μg of plasmid DNA released from 50 wt% DNA matrix coatings on day 3 was diluted to 1 μg per well and was complexed with Lipofectamine™ before introduction to cell culture. The transfection result of DNA matrix coatings (13,000 ± 5,000 relative luminescence/mg total protein) was equivalent to 60% gene delivery efficiency, in comparison with unencapsulated, stock plasmid DNA of same amount. (D) This value was compared with the % gene delivery efficiency obtained by DNA NP coating. (Grey bars = 3 days, black bars = 7 days, n = 3) *denotes statistical difference between the test groups and the untreated RAW 264.7