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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Differentiation. 2009 Oct 23;79(2):111–119. doi: 10.1016/j.diff.2009.09.004

Figure 5.

Figure 5

CD44 splicing is not altered following fiber cell removal. rt-PCR analysis of CD44 expression in the whole lens and the lens epithelium after lens fiber extraction using primers that amplify across the major region of CD44 alternate splicing. The canonical form of CD44 which does not utilize the variable exons gives a product of 180 bp (arrow). The differences in CD44 level in the lens epithelium following surgery were then determined by quantitative real time rt-PCR by the ΔΔcT method and are expressed as fold differences in expression level from samples collected immediately after surgery (0 hr). All of the post surgery samples were found to be significantly different than the 0 hr time point with p≤0.02. The conventional rt-PCR was controlled by performing controls lacking reverse transcriptase (-rt) and amplification of the samples with primers for β2-microglobulin (β2MG). M- marker; L- entire adult mouse lens; 12 hr- twelve hours following fiber cell extraction; 24 hr- 24 hours following fiber cell extraction; 48 hr- 48 hours following fiber cell extraction.