Table 2. The recruitment of RNA PolII and ERα on the pS2 and PR promoters in the presence of estrogen and bortezomib corresponds to the transcriptional response.

MCF7 cells were treated for 30 minutes with 30 nM bortezomib followed by 10 nM E2 for 24 hours. Cells were fixed, lysed, sonicated, and immunoprecipitated with antibodies for RNA polymerase II (RNA PolII), ERα, and IgG as a specificity control. Data are presented as the mean percent input +/- the standard error of the mean of three independent experiments.

		RNA level	RNA PolII	ERα	Ig G
Gene	Treatment	fold change	Promoter occupancy (relative ChIP value [% input])
PR
	EtOH	1.15 +/- 0.29	0.17 +/- 0.02	0.11 +/- 0.01	0.04 +/- 0.00
	B	2.93 +/-0.90	0.17 +/- 0.04	0.06 +/- 0.01	0.03 +/- 0.01
	E2	30.40 +/- 9.70	1.41 +/- 0.15	0.18 +/- 0.02	0.04 +/- 0.01
	E2/B	9.08 +/- 3.78	0.32 +/- 0.06	0.06 +/- 0.01	0.04 +/- 0.01
pS2
	EtOH	1.10 +/- 0.31	0.40 +/- 0.04	0.94 +/- 0.19	0.03 +/- 0.00
	B	0.50 +/- 0.14	0.27 +/- 0.09	0.77 +/- 0.57	0.03 +/- 0.00
	E2	25.37 +/- 0.91	0.94 +/- 0.09	3.97 +/- 0.41	0.04 +/- 0.01
	E2/B	80.54 +/- 10.76	0.68 +/- 0.17	3.09 +/-1.34	0.03 +/- 0.01