Fig. 3.
Bz-423 activates Bak and Bax. (A) MEFs were pre-treated with MnTBAP (100 μM) or vitamin E (100 μM) for 30 min prior to treatment with Bz-423 (12 μM, 12 h) and cell fractionation. Cytosolic or mitochondrial Bax was detected by immunoblot. The black vertical line indicates where non-adjacent lanes from the same gel were used to compile this figure. (B) MEFs were treated with vehicle or Bz-423 (12 μM, 12 h) followed by detection of activated N-terminal Bax or Bak by imunofluorescence microscopy.