Fig. 6.

CHX inhibits Bz-423 induced apoptosis. (A) Following pre-treatment (30 min) with CHX (1 μg/mL, dashed line) or vehicle (solid line), MEFs were incubated with Bz-423 and superoxide production (1 h) was monitored with DHE. (B) MEFs were pre-treated with CHX (1 μg/mL) or vehicle for 30 min prior to incubation with Bz-423 (12 μM, 12 h), fractionated as indicated, and immunoblotted to detect cytochrome c and Bax. (C) Following pre-treatment with CHX (1 μg/mL, dashed line) or vehicle (solid line), MEFs were incubated with Bz-423 and cell viability (24 h) determined by PI exclusion. P < 0.02 for vehicle vs CHX at [Bz-423] > 10 μM.