Fig. 7.

Bz-423 activates JNK. (A) Lysates prepared from MEFs treated with Bz-423 (10 μM) were immunoblotted to detect total and phosphorylated JNK. (B) Following treatment (2 h) with the indicated concentrations of Bz-423 (in μM), total cellular lysates were immunoblotted for total and phospho-p38. (C) Lysates prepared from MEFs treated with Bz-423 (10 μM) were immunoblotted to detect total and phosphorylated c-Jun and ATF2. (D) MEF were pre-treated with MnTBAP (100 μM), vitamin E (100 μM), or vehicle for 30 min prior to treatment with Bz-423 (10 μM, 2 h). Whole cell lysates were immunoblotted for JNK and phospho-JNK expression. The black vertical line indicates where non-adjacent lanes from the same gel were used to compile this figure. (E) Following pre-treatment with SP600125 (10 μM, dashed line) or vehicle (solid line), MEFs were incubated with Bz-423 and viability (24 h) was determined by PI exclusion. P < 0.01 for vehicle vs SP600125 at [Bz-423] > 8 μM. (F) Following pre-treatment with SP600125 (10 μM, dashed line) or vehicle (solid line), MEFs were incubated with Bz-423 and superoxide was detected by DHE (1 h).