Table 3.
Secondary metabolism genes up-regulated in Brunfelsia flowers after opening
| Systematic name | Average fold change (range) | Annotation | Function |
| STMHW02 | 5.39 (3.246–10.18) | (P15004) Suberization-associated anionic peroxidase 2 precursor (TMP2) | Suberanization |
| STMCL66 | 5.271 (4.096–7.023) | (Q9M527) Phenylcoumaran benzylic ether reductase homologue Fi2 | Lignification |
| STMDQ29 | 4.608 (3.016–9.118) | (Q41242) Alcohol dehydrogenase ADH | |
| STMCC79 | 3.475 (2.195–4.905) | (Q42958) Catechol O-methyltransferase (EC 2.1.1.6) | Lignification |
| STMJL95 | 3.387 (1.797–5.991) | (Q8H9B6) Caffeoyl-CoA O-methyltransferase (trans-caffeoyl-CoA 3-O-methyltransferase) (CCoAMT) | Lignification |
| STMIC60 | 3.361 (2.379–4.822) | (Q42945) Caffeoyl-CoA O-methyltransferase 6 (trans-caffeoyl-CoA 3-O-methyltransferase 6) (CCoAMT-6) | Lignification |
| STMCL31 | 3.037 (0.936–5.418) | (Q9AVG9) S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase | Scent/enzymatic activity that catalyses the formation of methylsalicylate from salicylic acid |
| STMER77 | 3.023 (1.58–7.432) | (P15004) Suberization-associated anionic peroxidase 2 precursor (TMP2) | Suberanization |
| STMCK29 | 2.867 (2.037–4.372) | (Q9XH50) 1-D-Deoxyxylulose 5-phosphate synthase | Terpenoids/IPP synthesis |
| STMCY65 | 2.745 (1.409–5.622) | (O82676) 1-Deoxyxylulose 5-phosphate synthase | Terpenoids/IPP synthesis |
| STMHZ50 | 2.586 (1.723–3.886) | (Q8H9B6) Caffeoyl-CoA O-methyltransferase (trans-caffeoyl-CoA 3-O-methyltransferase) (CCoAMT) | Lignification |
| STMEM71 | 2.4 (0.927–5.05) | (Q9MB73) Limonoid UDP-glucosyltransferase (limonoid glucosyltransferase) (limonoid GTase) (LGTase) | Terpenoids/glucosylation of limonoids |
| STMIN59 | 2.38 (1.399–4.517) | (Q8GVE3) Flavonoid 1–2 rhamnosyltransferase | Biosynthesis of flavonoids |
| STMEU71 | 2.365 (1.076–3.65) | (68418.m05207) O-Methyltransferase N-terminus domain-containing protein contains Pfam profile PF02409: | Lignification |
| STMIX91 | 2.322 (0.126–4.496) | (P30359) Cinnamyl-alcohol dehydrogenase (CAD) (EC 1.1.1.195) | Lignification |
| STMIJ60 | 2.267 (1.662–3.439) | (Q9SXJ0) Transcription factor Ntlim1 | Lignification/zinc binding |
| STMHR20 | 2.151 (1.657–3.4) | Adenosine monophosphate-binding protein 7 (AMPBP7_) | Acyl Co-A synthase/secondary metabolite biosynthesis |
| STMGM34 | 2.113 (1.5–3.42) | (Q42698) Geranylgeranyl pyrophosphate synthetase chloroplast precursor (GGPP synthetase) (GGPS) | Carotenoids |
| STMEP23 | 2.056 (1.16–3.063) | (Q41437) 3-Hydroxy-3-methylglutaryl-coenzyme A reductase 2 (HMG-CoA reductase 2) (HMG2.2) | Isoprenoid synthesis/mevalonate synthesis |
| STMCB66 | 2.054 (1.652–2.731) | (68417.m04881) LytB family protein contains Pfam profile: PF02401 LytB protein | Isoprenoid synthesis/enzyme |
| STMIV38 | 2.037 (0.684–5.894) | (P28554) Phytoene dehydrogenase chloroplast precursor (phytoene desaturase) | Carotenoids |
Genes were identified by heterologous hybridization of RNA from Brunfelsia flowers from D1 and D0 onto potato micrroarray, with a suggested function in the category of secondary metabolism. Genes were identified as significantly regulated (P = 0.05) at least one of the time points (D0 or D1) and have a >2-fold increase in D1 versus D0.