Fig. 3.
Stable transformation of C. gynandra. (A) Graft of a transformed shoot onto a wild-type scion allows seed set. (B–E) Regenerating hypocotyl and cotyledon explants after 4 weeks (B, D) or cotyledon explants at 8 weeks (C, E) of culture on regeneration medium containing 500 mg l−1 carbenicillin and 60 mg l−1 kanamycin. (B–D) Regenerants that show partial β-glucuronidase (GUS) staining. (E) Regenerants stained for GUS throughout the explant. Scale bars=2 mm.