Table 2.
Influence of Agrobacterium suspension media on pCaMV35S::uidA::Tnos transfer from LBA4404 containing SLJ1006 (Jones et al., 1992) into hypocotyls and cotyledons of C. gynandra
| Inoculum medium | MSO | A3 | SIM | ||||
| Regeneration medium | A5+C | A5+CK | A5+C | A5+CK | A5+C | A5+CK | |
| Hypocotyl | A | 20% (20) | 24% (46) | 15% (20) | 27% (44) | 90% (20) | 86% (42) |
| B | 10% (22) | 2% (46) | 5% (20) | 2% (46) | 40% (20) | 50% (46) | |
| Cotyledon | A | 43% (21) | 81% (31) | 48% (21) | 53% (38) | 100% (17) | 100% (26) |
| B | 47% (15) | 100% (16) | 60% (15) | 100% (16) | 100% (15) | 100% (6) | |
Two independent experiments (A and B) are reported in which three media were tested: MSO, A3, and SIM. The regeneration and selection medium was A5 and contained either 500 mg l-1 carbenicillin (A5+C) or 500 mg l−1 carbenicillin and 20 mg l−1 kanamycin (A5+CK). Details of media are reported in Materials and methods. Data are reported as the percentage of β-glucuronidase positive explants 8 d after incubation, with the total number of explants assessed in parentheses.