Table 5.
Callus production from hypocotyls and cotyledons on a matrix of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) Explants were scored for callus production after 5 weeks growth on regeneration medium A5 with 500 mg l−1 carbenicillin and 60 mg l−1 kanamycin sulphate. The effect of varying concentrations of BAP and NAA on callus production was assessed. Data are presented from two experiments as the percentage of explants that produced callus, and the total number of calli assessed in each experiment are in parentheses.
| Hypocotyls | BAP 0.5 mg−1 | BAP 1.0 mg l−1 | BAP 2.0 mg l−1 |
| NAA 0.1 mg l−1 | 53% (40) | 40% (40) | 28% (40) |
| 45% (40) | 40% (40) | 73% (40) | |
| NAA 0.5 mg l−1 | 28% (40) | 30% (40) | 40% (40) |
| 18% (40) | 35% (40) | 63% (40) | |
| NAA 1.0 mg l−1 | 15% (40) | 20% (40) | 28% (40) |
| 10% (40) | 25% (40) | 43% (40) | |
| NAA 1.5 mg l−1 | 5% (40) | 25% (40) | 15% (40) |
| 3% (40) | 50% (40) | 18% (40) | |
| Cotyledons | BAP 0.5 mg−1 | BAP 1.0 mg−1 | BAP 2.0 mg l−1 |
| NAA 0.1 mg 1−1 | 8% (24) | 15% (13) | 28% (25) |
| 31% (19) | 53% (19) | 89% (19) | |
| NAA 0.5 mg 1−1 | 0% (13) | 84% (19) | 15% (13) |
| 74% (19) | 20% (20) | 20% (39) | |
| NAA 1.0 mg 1−1 | 47% (19) | 68% (19) | 31% (19) |
| NAA 1.5 mg 1−1 | 12% (8) | 20% (10) | 16% (19) |
| 10% (20) | 5% (20) | 0% (6) |