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. 2010 Feb 22;61(5):1495–1508. doi: 10.1093/jxb/erq015

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Expression patterns of SPT reporter gene constructs in Arabidopsis. (A–G) Expression driven by the B. oleracea promoter pBoSPT-2660:GUS. (A) Inflorescence (transverse section), showing strong expression in regions of young flower primordia that will develop as stamens and carpels (floral stage indicated). (B) Gynoecium at stage 8 (transverse section) with strong medial expression (arrows). (C) Gynoecium at stage 11 (transverse section), with septum (sp) staining, and new expression in outer medial and lateral regions (arrowheads). (D) Gynoecium at stage 12 (longitudinal section), with staining in the transmitting tract (tt) and ovules (ov). Ovary wall staining is indicated by arrowheads. (E, F) Developing fruits (transverse sections) at stages 14–15 (E) and 17A (F), with staining in ovules (ov) at the earlier stage (E), but no staining visible in valve margins (arrows) where the dehiscence zone will arise. (G) Anther at stage 10–11 (transverse section), with staining in the tapetum (tp). (H–K) Expression driven by pSPT-1262:GUS, and pSPT-1262mutE1:GUS. (H, I) Buds at stage 12 (whole mounts, some sepals, petals, and stamens removed), showing that expression occurs in the carpel margins (arrows) in the normal –1262 construct (H) but not in the mutated version (I). Expression is visible in the stigma (sg) of both constructs. (J, K) Siliques at around stage 13 (transverse sections) show ovule (ov) expression in both (J, K), but in the valve margins only in the unmutated version (arrows) (J). (L–Q) Expression of the full-length promoter (pSPT-6253:GUS) in wild-type and indehiscent-12 mutant plants. Expression occurs in the valve margins of wild-type plants (arrows) but not in ind-12 plants. Expression in developing ovules and seeds is present in both. (L, M) Young siliques of stage 14 flowers (whole mounts, some sepals, petals, and stamens removed); (N, O) maturing siliques at stage 17A (whole mounts); (P, Q) maturing siliques at stage 17A (transverse sections).