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. 2010 Feb 22;61(5):1495–1508. doi: 10.1093/jxb/erq015

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 6. — Details of site directed mutagenesis of elements at three locations in the SPT promoter region. (A) Putative Auxin Response Elements (AuxREs), and nearby E-boxes (or variants), were individually mutated (indicated by X). They were generated in a full-length 1262 bp promoter GUS fusion, or in truncated or deleted versions of it. Expression patterns were recorded in the indicated number of independent transformants that showed any staining. (B) AuxREs and E-boxes in the wild-type sequence (boxed, upper sequences), and the mutational changes made (lower sequences). *Anti-sense sequence.