Table 1.
Expression patterns of 6253 bp of the SPT promoter region fused to the GUS reporter gene (first data column)
| bp upstream of full-length cDNA | −6253 | −2217 | −1592 | −1262 | −1203 | −357 | −313 | −260 | −221 | −180 |
| No. transformants | 21 | 29 | 11 | 13 | 9 | 20 | 8 | 11 | 17 | 21 |
| No. with staining | 16 | 25 | 7 | 10 | 6 | 13 | 6 | 9 | 14 | 12 |
| Enhancer regiona | ||||||||||
| −2217 to –6253 | ||||||||||
| Valve* | +++ | − | − | − | − | − | − | − | − | − |
| Endocarp | +++ | − | − | − | − | − | − | − | − | − |
| Petal blade* | +++ | − | − | − | − | − | − | − | − | − |
| Nectary | +++ | − | − | + | − | − | − | − | − | + |
| Receptacle | +++ | − | − | − | − | − | − | − | − | − |
| Hypocotyl/cotyledon | ++++ | ND | ND | − | ND | ND | ND | ND | ND | − |
| Pith* | +++++ | − | − | − | − | − | − | − | − | − |
| Hydathode | ++++ | − | − | − | − | − | − | − | − | − |
| Stomate | +++ | − | − | − | − | − | − | − | − | − |
| Enhancer regiona | ||||||||||
| −1 to –221 | ||||||||||
| Dehiscence zone* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + |
| Funiculus/ovule* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + |
| Tapetum* | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++ | +++ | ++ | + |
| Connective* | ++++ | +++ | ND | +++ | +++ | ++ | + | + | + | + |
| Root tip epidermis* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | + |
| SAM* and IM* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ++ | + |
| Developing gynoecium* | ++++ | +++ | ND | +++ | +++ | ++ | + | + | + | − |
| Septum/transtract* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | ++ | − |
| Stigma* | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | − |
| Stomium* | +++++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | − |
| Root tip stele | ++++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | + | − |
| Leaf vasculature* | ++++ | ND | ND | ND | ND | ND | ++ | − | − | − |
| Leaf primordium | ++++ | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| Embryo | ++++ | ND | ND | +++ | ND | ND | ND | ND | ND | ND |
| Silencing regions (various) | ||||||||||
| Petal claw | + | ++++ | ++++ | ++++ | ++++ | ++++ | +++ | +++ | ++ | − |
| Basal sepal margin | − | +++ | ND | +++ | +++ | +++ | ++ | ++ | ++ | − |
| Basal leaf margin | + | +++ | +++ | +++ | +++ | +++ | +++ | ++ | ++ | − |
| Apex of filament | − | − | +++ | +++ | ND | +++ | ND | ND | ++ | +++ |
| Leaf epidermis | − | − | − | − | − | ND | ++ | ++ | ++ | − |
| Root epidermis | − | − | − | − | − | − | − | − | +++ | − |
Also shown are staining patterns in a series of 5’ truncations of this construct, with the location of the 5’ end of the truncation shown at the head of each column. Tissues are organized into categories depending on the deduced location of their specific enhancer elements. Relative staining levels are indicated by the number of + symbols; no detectable staining is indicated by a – symbol. ND: not determined.
a
Transcripts detected by in situ hybridization (Heisler et al., 2001) shown by an asterisk.