Isolation of MaMADS-box genes from Cavendish banana (cultivar Grand Nain). (A) Putative proteins alignment of MaMADS1–6 sequences isolated from banana fruit and the similarity among them. The gene sequences were deposited in http://www.ncbi.nlm.nih.gov/BLAST as EU869307, EU869306, EU869308, EU869309, EU869310, and EU869311, respectively. (B) Phylogenetic analysis of MaMADS1–4 with other SEP3 clade genes. The analysis was performed on the full-lengths of the individual genes. The SEP3 motif for each of the genes is presented on the right. The genes included in this alignment are: AoAOM1 (AAQ83834), SISEP3 (BAD10945.1), LItuAGL9 (AAX15920.1), PaAGL9.1 (AAX15923.1), HcSEP1 (BAC80253.1), PsM6 (AAX69068), DcSEP1 (AAZ95252.1), AdMADS (CAA48859.1), SaMADSD (CAA69916.1), PsMADS (CAA11258.1), PhFBP26 (AAF19164.1), NsMADS3 (AAD39034.1), LeTM5 (AAP57413.1), EcAGL9 (AAX15918.1), DeCDM44 (AAO22982.1), BpMADS (CAB95648.1), DEFH200 (CAA64743.1), PtMADS6 (AAO49811.1), AtSEP (AAT46095.1), VvMADS4 (AAM21344.1), AmDEFH72 (CAA64742.1). (C) Phylogenetic analysis of MaMADS5 with other AG genes. The existence of AG motifs for all the genes is shown. The genes included in this alignment are: DtSEEDSTICK (AAY86365.1), DnMADS2 (ABQ08574.1), AvMADS (BAD83772.1), HoMADS1 (AAF08830.2). (D) Phylogenetic analysis of MaMADS6 with other PI genes. The existence of PI motifs for all the genes are shown. The genes included in this alignment are: AoPI (ABB92623.1), P9PI (AAV28175.1), PI10 (AAV28490.1), HPI2 (AAD22494.2), PI1 (ABG90945.1). Lines underneath the genes mark the genes isolated from banana. Stars and dots indicate identity and similarity, respectively.