Fig. 5.

Effect of pH on currents in Xenopus oocytes expressing HvALMT1. Xenopus laevis oocytes injected with HvALMT1 cRNA or water as a control were incubated overnight in 10 mM malate–BTP. The currents across the oocyte membrane were recorded using the two-electrode voltage clamp technique, while the oocytes were bathed in either 10 mM MES–BTP (MES), 10 mM chloride–BTP (Cl), or 10 mM malate–BTP (malate) at external pH 4.5, 5.5, or 7.5. Shown is the current across the membrane of oocytes clamped at 60 mV and –140 mV. Error bars show the SEM (n=3).