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. Author manuscript; available in PMC: 2010 Mar 12.
Published in final edited form as: Scand J Immunol. 2008 Apr 10;67(6):603–609. doi: 10.1111/j.1365-3083.2008.02102.x

Figure 1.

Figure 1

Identification of antibodies to Trk receptors in the sera of patients with Chagas’ disease. (A) Screening of antibodies to TrkA, TrkB or TrkC by ELISA in 25 chagasic sera and 29 non-chagasic sera. Chagasic sera were also screened with five other growth factor receptors (p75NTR, GFR-α1, c-Ret, TGF-βR-II and FGFR). (B) Titration by ELISA of one non-chagasic and one chagasic (megacolon) chagasic serum against TrkA, TrkB and TrkC. (C) Immunoblotting of PC12wt and PC12nnr5 cell lysates with commercial rabbit antibody to TrkA (α-TrkA) and with chagasic (megacolon) TrkA-affinity-purified antibodies (ATA). Blot shows reaction (arrows) of the antibody with the fully glycosylated (140 kDa) and precursor (110 kDa) TrkA isoforms. (D) Fluorescence microscopy of PC12wt cells (a) and PC12nnr5 cells (b) immunostained with ATA (IND phase) followed by Alexa Fluor 594-labelled goat anti-human antibody. Panels (c) and (d) show the reaction of Alexa-labelled second antibody with PC12wt cells without ATA and with chagasic affinity-purified PDNF antibodies. ×400. (E) Cross-reaction of TrkA-, TrkB- and TrkC-affinity purified ATA with Trks A, B and C, but not with c-Ret by ELISA. Antibodies were isolated from IND IgG [same donor as in (D)] on solid-phase TrkA (ATA/TrkA), TrkB (ATA/TrkB) and TrkC (ATA/TrkC), and reacted by ELISA with TrkA, TrkB, TrkC and c-Ret.