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ATA form dimers and do not react with Trk-bound carbohydrate. (A) Analysis of ATA (IND) for the presence of dimers by non-reducing SDS-PAGE on 5% gels visualized on immunoblotting with goat anti-human IgG antibody followed by HRP-conjugated secondary antibody. (B) Reaction by ELISA of ATA isolated on solid-phase TrkA (ATA/TrkA) or TrkC (ATA/TrkC) with recombinant TrkA expressed in Escherichia coli (Bact-TrkA) or myeloma cells (Mam-TrkA), and with negative control GFRα1 receptor.
