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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Eur J Immunol. 2010 Jan;40(1):142–152. doi: 10.1002/eji.200939618

Figure 3.

Figure 3

Induction of Foxp3+ by TGF-β is independent upon BMP receptor signal. (A) Foxp3 expression was determined in CD4+ cells treated with medium, TGF-β, and both TGF-β and Noggin treated CD4+ cells. Data are representative of three independent experiments. (B) The suppressive activity of these cells was analyzed by a standard suppressive assay as described in Fig 1B. (C) The effects of Noggin on the Smad1 activation of BMP-4 on CD4+ cells. Naïve CD4+ cells were stimulated with BMP-4 with or without Noggin for 2 hours and Smad1 and phosphorylated Smad1 expression were determined by western blot. Experiments were repeated twice with similar results.