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. Author manuscript; available in PMC: 2010 Mar 12.
Published in final edited form as: Small. 2009 Nov;5(21):2453–2459. doi: 10.1002/smll.200900467

Figure 1.

Figure 1

A) Map of E. coli expression vector plasmid pET32 harboring eGFP-gp16 with His-tag. B) Schematic illustration showing the structure of the nanomotorwith eGFP-gp16. C) eGFP-gp16 purified by His-tag affinity chromatography (10% SDS-PAGE). Lane 1: Molecular-weight protein marker. Lanes 2 and 3: E. coli BL21(DE3) with the pET32-eGFP-gp16 uninduced and induced by IPTG, respectively. Lanes 4 and 5: soluble cell extracts containing over-expressed eGFP-gp16 before and after passage through an affinity chromatography column, respectively. Lane 6: purified eGFP-gp16.