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Binding activity of eGFP-gp16 to DNA observed by TIRF imaging microscopy. Cy5-poly dT was incubated inside a microchamber in the presence (A) or the absence (B) of immobilized eGFP-gp16on the surface. Engineered-gp16 was used as a control for DNA binding (C). The image was taken from both eGFP and Cy5 emission channels.
