Skip to main content
. Author manuscript; available in PMC: 2011 Mar 5.
Published in final edited form as: Cell Stem Cell. 2010 Mar 5;6(3):265–278. doi: 10.1016/j.stem.2010.02.002

Figure 4.

Figure 4

Clonal analysis of HSC subtypes using single-cell transplantation.

(A) Tracking of eight individual lower- and upper-SPKLS clones showing overall hematopoietic chimerism in recipient mice (engraftment; top panels in grey) and lineage distribution of test cells 12-weeks post-transplant. (B) Analysis of overall engraftment and lineage contribution of lower- and upper-SPKLS cells also fractionated with respect to CD150. (C) Distribution of lineage contribution 12-weeks after transplant from mice transplanted with single HSCs with the indicated phenotypes. (D) Average level of hematopoietic chimerism from single-cell transplantation experiments. (E) Lineage contribution measured 12 weeks after secondary transplantation of cells derived from primary mice reconstituted with a single HSC. Bone marrow from each clone was tested in four new recipients. All clones met the assigned threshold of at least 0.1% overall contribution to all 4 secondary recipients at 12-weeks post-transplant, with the exception of USP-3, in which 3/4 recipients fell just below the assigned threshold (see also Figure S3).