Figure 1.

In vivo methylation of CpG dinucleotides of the rho promoter in 5-day and adult chicken erythroid cells using the bisulfite conversion technique. Arrows indicate methylated cytosines. Positions indicated are relative to the transcription start site (A). Cytosines that are not associated with CpG dinucleotides (sequence shown in B) have all been converted to thymidines in both 5-day and adult erythroid cells. (B) ρ-Globin gene promoter sequence. Arrows indicate methylated cytosines that are clearly seen in A (data not shown for CpG dinucleotide at position −15). Primers R and F indicate the sequence of ρ-globin gene promoter used for designing internal primers and for sequencing.