Figure 3.

Mobility shift assay for MeCPC binding to methylated CG11 probe (M-CG11). 0.6 ng of methylated CG11 end-labeled probes were mixed with 6 μg of HeLa cell nuclear extract and varying amounts of mock-methylated or methylated rho 235 promoter DNA fragment. The reaction mixture was electrophoresed in a 1.5% agarose gel, and complexes were analyzed by autoradiography. This autoradiogram illustrates the effective competition of MeCPC binding to M-CG11 probe by methylated rho 235 but not by mock-methylated rho 235 (M-rho 235).