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. 1997 Dec 9;94(25):13724–13729. doi: 10.1073/pnas.94.25.13724

Figure 4.

Figure 4

Mobility shift assay for the presence of MeCPC in chicken erythroid cell or control HeLa cell nuclear extracts. End-labeled, methylated CG11 probe (M-CG11) but not mock-methylated CG11 (CG11) forms a complex with erythroid cell nuclear extract that has similar mobility as seen with HeLa cell nuclear extract (A). Similar results were seen in 5-day chicken erythroid cell nuclear extract (data not shown). (B) Binding of MeCPC in chicken erythroid cell nuclear extract to methylated rho 235 (M-rho 235) probe. End-labeled M-rho 235 but not mock-methylated rho 235 forms a complex with erythroid cell nuclear extract. This complex was competed effectively by an excess of unlabeled methylated but not mock-methylated rho 235.