Table 3.
Results of transplantations from CD34−, CD34dim, and CD34+ fractions of AML into immunodeficient mice
| Sample | Time, weeks | CD34− |
CD34+ |
CD34dim |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dose × 106 | MNC dose × 106 | Mice with AML/mice total | Dose × 103 | MNC dose × 106 | Mice with AML/mice total | Dose × 103 | MNC dose × 106 | Mice with AML/mice total | ||
| 1 | 12-13 | 24 | 24 | 2/2* (2.8%) | 7.8 | 5.5 | 0/3† | NA | NA | NA |
| 1 | 12 | 5 | 5 | 0/2 | ||||||
| 2 | 12 | 21 | 21 | 2/2* (1.2%) | NT | NT | NT | NA | NA | NA |
| 7 | 9 | ‡ | ‡ | ‡ | 2.5 | 5.2 | 0/1 | 11 | 9.2 | 0/1 |
| 8 | 12 | 5.4 | 5.4 | 2/3* (0.1%) | 4.2 | 19 | 0/1† | 5 | 5.2 | 0/2 |
| 9 | 13-15 | 13 | 13 | 4/4* (68%) | 8.4 | 15 | 0/1 | 400 | 64 | 0/1 |
| WT1 | 9 | 6 | 6 | 1/1 (60%) | 3.2 | 6 | 0/1 | NA | NA | NA |
NA indicates not applicable as these samples lack the CD34dim population; NT, not tested; and WT1, wild-type NPM AML sample 1. The figures in parentheses indicate the mean percentage of AML in the mouse bone marrow.
*
Sorted human cells from mouse bone marrow were shown to contain mutated NPM by quantitative PCR.
†
Normal multilineage engraftment observed; the DNA from the grafts contained wild-type NPM.
‡
The LICs from sample 7 are found in the CD34− fraction (Table 4).