Figure 1.

Immunoblots of cytoskeletal proteins of reticulocytes and erythrocytes. (A) Intact cells: Western blots of SDS-PAGEs of total cellular protein prepared from reticulocytes and erythrocytes probed with antibodies against the indicated proteins. Note the absence of tubulin and the decreased expression of tropomyosin, myosin, actin and adducin in erythrocytes. (B) Ghosts: Western blots of SDS-PAGEs of ghosts prepared from reticulocytes and erythrocytes probed with antibodies against the indicated proteins. Because ghosts of both reticulocytes and erythrocytes do not contain any tubulin, there is no corresponding panel for tubulin in panel B. Note the decreased expression of tropomyosin and myosin in the erythrocyte membranes but the similar level of actin and adducin. (C) Western blots of SDS-PAGEs of the cytosol and the membrane were probed with anti-actin or anti-adducin antibody. Note actin and adducin are present in both the cytosol and the membrane fraction of reticulocytes but are only present in the membrane of erythrocytes.