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Interaction of p53 and DJ-1 protein in KG1a cells. Whole-cell lysates from KG1a cells were immunoprecipitated with an anti–DJ-1 antibody and protein-A beads, or alternatively, with anti–TWIST-1 or Stro-1 antibodies (as positive [+] and negative [−] controls, respectively). The protein complexes brought down by anti–DJ-1 or anti-TWIST antibody contained p53, consistent with protein-protein interactions of p53 and DJ-1. One of 4 identical experiments is shown.
