Table 1. Specificity of rhinocerase towards Arg-AMC.
| Activity (Substrate) | Enzyme | Km a (µM) | Vmax a (s−1) | Kcat a (s−1) | Kcat/Km x 103a (µM−1s−1) |
| Serine protease (Arg-AMC) | Trypsin | 39.19±1.19 | - | 114.39±3.6 | 2919.13±6.7 |
| Thrombin | 60.6±5.92 | 0.43±0.03 | - | - | |
| Venom | 6.66±0.57 | 0.07±0.003 | - | - | |
| Rhinocerase | 80.05±2.02 | - | 214.52±3.9 | 2680.15±20.87 |
a
The values are mean ± S.D. (n = 3).
100 µg of rhinocerase were incubated with different concentrations of Arg-AMC at 37°C for 7 minutes. The amount of AMC released was measured at an excitation wavelength of 366 nm and an emission wavelength of 460 nm at time intervals up to 7 minutes. 100 µg of B. g. rhinoceros venom, 100 µg of trypsin and 10 units of thrombin were also used at the same experimental conditions for comparison. Kinetic parameters were determined from Lineweaver-Burk plots.