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. Author manuscript; available in PMC: 2010 Mar 12.
Published in final edited form as: Hum Gene Ther. 2005 Sep;16(9):1097–1109. doi: 10.1089/hum.2005.16.1097

FIG. 3.

FIG. 3

Functional assay of chimeric pRNA/siRNA(GFP) by transfection. (A–C) Fluorescence microscopy images showing the silencing of GFP gene by transfection. (A) Dose-dependent silencing of GFP gene by chimeric pRNA/siRNA(GFP) (left column). A mutant pRNA/siRNA (right column) served as negative control. (B) GFP expression of cells transfected with various RNAs: (a) no RNA; (b) synthesized double-stranded siRNA(GFP); (c) double-stranded siRNA(LacZ) control; (d) pRNA/siRNA(GFP); (e) pRNA/siRNA(mutant); (f) pRNA vector alone. (C) Comparison of the performance of (a) chimeric pRNA/siRNA(GFP) and (b) conventional double-stranded siRNA(GFP) at the same molar concentration; (c) control with no siRNA treatment. (D) Northern blot to examine the effect of chimeric pRNA/siRNA(GFP) on GFP mRNA level after transfection. Lanes 1 and 2 show the effects of two different constructs of pRNA/siRNA(GFP); lane 3, double-stranded siRNA; lane 4, cells without RNA treatment. rRNA was used as loading control.