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. Author manuscript; available in PMC: 2010 Mar 12.
Published in final edited form as: Hum Gene Ther. 2005 Sep;16(9):1097–1109. doi: 10.1089/hum.2005.16.1097

FIG. 5.

FIG. 5

Apoptosis and cell death induced by transfection of chimeric pRNA harboring siRNA targeting survivin. (I) Breast cancer MCF-7 cells were transfected with pRNA/siRNA(survivin) and apoptosis was monitored by PI–annexin V double-labeling followed by flow cytometry. Cells in the bottom right quadrant represent apoptotic cells. (II) Breast cancer cells (MDA-231) and prostate cancer cells (PC-3) were transfected with 20 pmol of pRNA/siRNA(survivin) in 24-well plates and images were taken 24 hr after transfection. The mutant pRNA/siRNA was transfected in parallel as a negative control.