FIG. 7.

Specific delivery of chimeric pRNA/siRNA by CD4 receptor. (I) Green circles corresponding to the binding of FITC-labeled pRNA dimer containing CD4-binding aptamer to lymphocytes were shown by confocal microscopy (a) and the entry of RNA was shown as a green spot inside the cell (d). Texas Red-labeled transferrin was used as a positive control of internalization (b). No binding was observed in the control cell line without CD4 expression (c). (II) Incubation of RNA dimer led to the specific suppression of cell viability, as measured by trypan blue exclusion assay. Three different cell lines with no, low, or high CD4 expression level were incubated with the pRNA/siRNA(survivin)-pRNA/aptamer(CD4) complex in the presence or absence of IL-7.