Table 1.

Animal Trial in Epidermal Cancer Therapy^a

Treatment group	No. of cancerous animals/no. of animals tested
1: No pRNA	4/8
2: Dimer [folate-pRNA(B-a′) + pRNA/siRNA(survivin)(A-b′)]	0/7
3: Dimer [pRNA(B-a′) + pRNA/mutant-siRNA(A-b′)]	7/8
4: Dimer [pRNA(B-a′) + pRNA/siRNA(survivin)(A-b′)]	6/8

^aKB cells were maintained in folate-free medium RPMI 1640.

Cells were preincubated with pRNA complex for 3 hr before being used for animal injection. After rinsing twice with PBS containing 10 mM MgCl₂, cells were collected into a centrifuge tube. Each mouse was inoculated with 2.5 × 10³ cells in 0.1 ml of medium. Shown are the results of in vivo testing of mice receiving tumor xenografts along with the chimeric pRNA complex. One mouse in group 2 produced a plaque within 1 week, much earlier than any of the other mice in any group, and therefore given these special circumstances it was treated as an outlier and there are seven mice recorded for group 2 instead of eight.