Figure 2. YfiN is a diguanylate cyclase.

A) A truncated YfiN lacking the transmembrane and periplasmic parts has DGC activity in vitro. C-di-GMP production was observed on TLC plates over time against a positive control (DgcA). The c-di-GMP peak is marked with an arrow. B) YfiN binding of c-di-GMP. Elution and wash fractions from YfiN purification were run on an HPLC column and the absorbance at 254 nm plotted. The elution fraction contains a large c-di-GMP peak, presumably bound by YfiN, and not seen in the wash fraction.