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. 2010 Mar 12;6(3):e1000806. doi: 10.1371/journal.ppat.1000806

Figure 2. Ras1 plays a major role in GlcNAc induction.

Figure 2

A. Switching frequencies (Switch. Freq.) of wild type (WT) and ras1/ras1 cells grown in a glucose liquid medium at 25°C for 7 days and then plated on glucose agar and GlcNAc agar at 25°C. The colony numbers assessed for phenotype varied between 600 and 1,000 under varied conditions. B. Colonies of the inducible strain WT+MET3p-RAS1V13 grown on glucose at 25°C agar in the presence (repressing condition) or absence (activating condition) of methionine and cysteine. White cells from glucose agar (repressing condition) were plated. C. Switching frequencies of cells of the control strain WT+vector and the RAS1V13 overexpression strain WT+MET3p-RAS1V13 grown at 25°C in glucose liquid medium for one day and then plated on glucose or GlcNAc agar at 25°C. The colony numbers assessed ranged between 250 and 400. D. Examples of colonies on glucose and GlcNAc agar under activating conditions. E. Examples of cells from colonies on glucose and GlcNAc agar at 25°C under activating conditions. The scale bars in panel E represents 10 µm. Switching frequencies were measured after 5 days of growth at 25°C on agar.