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. 2010 Mar 12;6(3):e1000806. doi: 10.1371/journal.ppat.1000806

Figure 3. The genes CDC35, PDE2, TPK1 and TPK2 play roles in GlcNAc induction of switching.

Figure 3

A. Switching frequencies (Switch. Freq.) of cells of the mutants cdc35/cdc35 and pde2/pde2 grown at 25°C in glucose liquid medium for 7 days (cdc35/cdc35) or 5 days (pde2/pde2), and then plated on glucose or GlcNAc agar at 25°C. The number of assessed colonies ranged between 200 and 450. B. Streaks of cells grown for 5 days of the parental control strain (WT), the mutant control strain pde2/pde2+vector and the inducible strain pde2/pde2+MET3p-PDE2 under inducing (-Met, -Cyc) and inducing (+Met, +Cys) condition. C. Switching frequencies of cells of the mutant control pde2/pde2+vector and the rescued strain pde2/pde2+MET3p-PDE2 grown in liquid glucose medium for 2 days and then plated on glucose and GlcNAc agar under activating conditions. The number of assessed colonies ranged between 350 and 450. D. Examples of colonies of pde2/pde2+vector and pde2/pde2+MET3p-PDE2 from panel C under activating conditions. E. Examples of cells from colonies in Figure D. F. Switching frequencies of parental control strains, the mutants tpk1/tpk1 and tpk2/tpk2 and the overexpression strains WT+ACTp-TPK1 and WT+ACTp-TPK2 grown in glucose medium for 5 days and then plated on glucose or GlcNAc agar. The number of assessed colonies ranged between 250 and 800 colonies.