Table 1. GlcNAc induction of white-to-opaque switching is enhanced at 37°C and in 1% CO_2.

Strain	Agar Medium, Temp	Air	1% CO2
		Switching frequency (%)	Switching frequency (%)
5314a	Glucose, 25°C	0.4±0.6	21.4±1.4
	37°C	<0.4	7.5±1.5
	GlcNAc, 25°C	26.7±1.2	100.0±0.0
	37°C	98.9±1.2	100.0±0.0
5314α	Glucose, 25°C	0.2±0.4	19.4±1.8
	37°C	<0.2	7.1±0.5
	GlcNAc, 25°C	22.4±3.3	100.0±0.0
	37°C	99.7±0.6	99.4±0.5

As described in Figure 1A, white cells were cultured for 48 hours in glucose liquid medium at 25°C, then plated onto glucose or GlcNAc agar, and incubated at 25 or 37°C in air or air containing 1% CO₂. Total colonies analyzed in each strain and condition varied between 150 and 400.