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. 2010 Mar 12;6(3):e1000811. doi: 10.1371/journal.ppat.1000811

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Martinez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Female C57BL/6 mice were depleted of NK cells with anti-NK1.1 antibodies (25 µg) on days −3 and 0 (+anti-NK1.1) or left untreated (Control), followed by infection with VV on day 0. (A) 48 h after infection, splenocytes were assayed for NK lytic activity on YAC-1 cells for 4 hr at different effector∶target ratios. Naïve splenocytes (Naïve) were used for comparison. The percentage of specific lysis is shown. (B) The ovaries were assayed for viral load. Data represents viral titer ± SD as pfu per ovary.