Table 2.
Repeated measures ANOVA of survival, deformities, and developmental time in long-toed salamander (A. macrodactylum) embryos
| Source of variation | MS | DF | F | P |
|---|---|---|---|---|
| Survival | ||||
| Treatment | 0.456 | 1 | 6.343 | 0.045 |
| Error | 0.072 | 6 | ||
| Time | 0.360 | 3 | 6.135 | 0.005 |
| Time X treatment | 0.308 | 3 | 5.259 | 0.009 |
| Error | 0.059 | 18 | ||
| Deformities | ||||
| Treatment | 1.145 | 1 | 7.659 | 0.033 |
| Error | 0.150 | 6 | ||
| Time | 0.392 | 3 | 4.682 | 0.014 |
| Time X treatment | 0.385 | 3 | 4.588 | 0.015 |
| Error | 0.084 | 18 | ||
| Developmental rate | ||||
| Treatment | 16.335 | 1 | 8.868 | 0.025 |
| Error | 1.842 | 6 | ||
| Time | 661.022 | 2 | 933.940 | <0.0001 |
| Time X treatment | 7.875 | 2 | 11.027 | 0.002 |
| Error | 0.708 | 12 |
A preliminary analysis indicated no block effects (i.e., no differences between temperature or other variables among blocks) so the block and error terms were pooled for remaining tests (28). Percent survival and percent deformities data were Arcsine transformed before analysis. Temperatures were taken on the four occasions when embryos were checked (Table 1). Student’s t tests revealed no significant temperature differences between treatments (T30 = 0.251, P = 0.804; mean (±SE) temperatures were 23.28°C (0.35) and 23.41°C (0.35) for the mylar and acetate regimes, respectively. MS, mean squares; DF, degrees of freedom; F, F statistic.